Concentration Assay:Article Title: A2B adenosine receptor signaling attenuates acute lung injury by enhancing alveolar fluid clearance in mice
Article Snippet: In subsets of experiments, mice were treated with the A2BAR antagonist PSB1115 (10 mg/kg i.p.; Tocris) or the A2BAR agonist BAY 60-6583 (2 mg/kg i.p.; Bayer Healthcare), the A1AR antagonist DPCPX (1 mg/kg i.p.; Tocris), the A3AR antagonist MRS1191 (1 mg/kg i.p.; Sigma-Aldrich), amiloride (10 –3 M, 300 μl intratracheally; Sigma-Aldrich), or zinterol (10 –7 M, 300 μl intratracheally; Tocris); or vehicle ( 13 ).
Article Title: A2B adenosine receptor signaling attenuates acute lung injury by enhancing alveolar fluid clearance in mice
Article Snippet: In subsets of experiments, mice were treated with the A2BAR antagonist PSB1115 (10 mg/kg i.p.; Tocris) or the A2BAR agonist BAY 60-6583 (2 mg/kg i.p.; Bayer Healthcare), the A1AR antagonist DPCPX (1 mg/kg i.p.; Tocris), the A3AR antagonist MRS1191 (1 mg/kg i.p.; Sigma-Aldrich), amiloride (10–3 M, 300 μl intratracheally; Sigma-Aldrich), or zinterol (10–7 M, 300 μl intratracheally; Tocris); or vehicle (13).
Enzyme-linked Immunosorbent Assay:Article Title: A2B adenosine receptor signaling attenuates acute lung injury by enhancing alveolar fluid clearance in mice
Article Snippet: In subsets of experiments, mice were treated with the A2BAR antagonist PSB1115 (10 mg/kg i.p.; Tocris) or the A2BAR agonist BAY 60-6583 (2 mg/kg i.p.; Bayer Healthcare), the A1AR antagonist DPCPX (1 mg/kg i.p.; Tocris), the A3AR antagonist MRS1191 (1 mg/kg i.p.; Sigma-Aldrich), amiloride (10 –3 M, 300 μl intratracheally; Sigma-Aldrich), or zinterol (10 –7 M, 300 μl intratracheally; Tocris); or vehicle ( 13 ).
Article Title: A2B adenosine receptor signaling attenuates acute lung injury by enhancing alveolar fluid clearance in mice
Article Snippet: In subsets of experiments, mice were treated with the A2BAR antagonist PSB1115 (10 mg/kg i.p.; Tocris) or the A2BAR agonist BAY 60-6583 (2 mg/kg i.p.; Bayer Healthcare), the A1AR antagonist DPCPX (1 mg/kg i.p.; Tocris), the A3AR antagonist MRS1191 (1 mg/kg i.p.; Sigma-Aldrich), amiloride (10–3 M, 300 μl intratracheally; Sigma-Aldrich), or zinterol (10–7 M, 300 μl intratracheally; Tocris); or vehicle (13).
MPO Assay:Article Title: A2B adenosine receptor signaling attenuates acute lung injury by enhancing alveolar fluid clearance in mice
Article Snippet: In subsets of experiments, mice were treated with the A2BAR antagonist PSB1115 (10 mg/kg i.p.; Tocris) or the A2BAR agonist BAY 60-6583 (2 mg/kg i.p.; Bayer Healthcare), the A1AR antagonist DPCPX (1 mg/kg i.p.; Tocris), the A3AR antagonist MRS1191 (1 mg/kg i.p.; Sigma-Aldrich), amiloride (10 –3 M, 300 μl intratracheally; Sigma-Aldrich), or zinterol (10 –7 M, 300 μl intratracheally; Tocris); or vehicle ( 13 ).
Article Title: A2B adenosine receptor signaling attenuates acute lung injury by enhancing alveolar fluid clearance in mice
Article Snippet: In subsets of experiments, mice were treated with the A2BAR antagonist PSB1115 (10 mg/kg i.p.; Tocris) or the A2BAR agonist BAY 60-6583 (2 mg/kg i.p.; Bayer Healthcare), the A1AR antagonist DPCPX (1 mg/kg i.p.; Tocris), the A3AR antagonist MRS1191 (1 mg/kg i.p.; Sigma-Aldrich), amiloride (10–3 M, 300 μl intratracheally; Sigma-Aldrich), or zinterol (10–7 M, 300 μl intratracheally; Tocris); or vehicle (13).
Activity Assay:Article Title: A2B adenosine receptor signaling attenuates acute lung injury by enhancing alveolar fluid clearance in mice
Article Snippet: In subsets of experiments, mice were treated with the A2BAR antagonist PSB1115 (10 mg/kg i.p.; Tocris) or the A2BAR agonist BAY 60-6583 (2 mg/kg i.p.; Bayer Healthcare), the A1AR antagonist DPCPX (1 mg/kg i.p.; Tocris), the A3AR antagonist MRS1191 (1 mg/kg i.p.; Sigma-Aldrich), amiloride (10 –3 M, 300 μl intratracheally; Sigma-Aldrich), or zinterol (10 –7 M, 300 μl intratracheally; Tocris); or vehicle ( 13 ).
Article Title: A2B adenosine receptor signaling attenuates acute lung injury by enhancing alveolar fluid clearance in mice
Article Snippet: In subsets of experiments, mice were treated with the A2BAR antagonist PSB1115 (10 mg/kg i.p.; Tocris) or the A2BAR agonist BAY 60-6583 (2 mg/kg i.p.; Bayer Healthcare), the A1AR antagonist DPCPX (1 mg/kg i.p.; Tocris), the A3AR antagonist MRS1191 (1 mg/kg i.p.; Sigma-Aldrich), amiloride (10–3 M, 300 μl intratracheally; Sigma-Aldrich), or zinterol (10–7 M, 300 μl intratracheally; Tocris); or vehicle (13).
Control:Article Title: A2B adenosine receptor signaling attenuates acute lung injury by enhancing alveolar fluid clearance in mice
Article Snippet: In subsets of experiments, mice were treated with the A2BAR antagonist PSB1115 (10 mg/kg i.p.; Tocris) or the A2BAR agonist BAY 60-6583 (2 mg/kg i.p.; Bayer Healthcare), the A1AR antagonist DPCPX (1 mg/kg i.p.; Tocris), the A3AR antagonist MRS1191 (1 mg/kg i.p.; Sigma-Aldrich), amiloride (10 –3 M, 300 μl intratracheally; Sigma-Aldrich), or zinterol (10 –7 M, 300 μl intratracheally; Tocris); or vehicle ( 13 ).
Article Title: A2B adenosine receptor signaling attenuates acute lung injury by enhancing alveolar fluid clearance in mice
Article Snippet: In subsets of experiments, mice were treated with the A2BAR antagonist PSB1115 (10 mg/kg i.p.; Tocris) or the A2BAR agonist BAY 60-6583 (2 mg/kg i.p.; Bayer Healthcare), the A1AR antagonist DPCPX (1 mg/kg i.p.; Tocris), the A3AR antagonist MRS1191 (1 mg/kg i.p.; Sigma-Aldrich), amiloride (10–3 M, 300 μl intratracheally; Sigma-Aldrich), or zinterol (10–7 M, 300 μl intratracheally; Tocris); or vehicle (13).
Staining:Article Title: A2B adenosine receptor signaling attenuates acute lung injury by enhancing alveolar fluid clearance in mice
Article Snippet: In subsets of experiments, mice were treated with the A2BAR antagonist PSB1115 (10 mg/kg i.p.; Tocris) or the A2BAR agonist BAY 60-6583 (2 mg/kg i.p.; Bayer Healthcare), the A1AR antagonist DPCPX (1 mg/kg i.p.; Tocris), the A3AR antagonist MRS1191 (1 mg/kg i.p.; Sigma-Aldrich), amiloride (10 –3 M, 300 μl intratracheally; Sigma-Aldrich), or zinterol (10 –7 M, 300 μl intratracheally; Tocris); or vehicle ( 13 ).
Article Title: A2B adenosine receptor signaling attenuates acute lung injury by enhancing alveolar fluid clearance in mice
Article Snippet: In subsets of experiments, mice were treated with the A2BAR antagonist PSB1115 (10 mg/kg i.p.; Tocris) or the A2BAR agonist BAY 60-6583 (2 mg/kg i.p.; Bayer Healthcare), the A1AR antagonist DPCPX (1 mg/kg i.p.; Tocris), the A3AR antagonist MRS1191 (1 mg/kg i.p.; Sigma-Aldrich), amiloride (10–3 M, 300 μl intratracheally; Sigma-Aldrich), or zinterol (10–7 M, 300 μl intratracheally; Tocris); or vehicle (13).
Isolation:Article Title: A2B adenosine receptor signaling attenuates acute lung injury by enhancing alveolar fluid clearance in mice
Article Snippet: In subsets of experiments, mice were treated with the A2BAR antagonist PSB1115 (10 mg/kg i.p.; Tocris) or the A2BAR agonist BAY 60-6583 (2 mg/kg i.p.; Bayer Healthcare), the A1AR antagonist DPCPX (1 mg/kg i.p.; Tocris), the A3AR antagonist MRS1191 (1 mg/kg i.p.; Sigma-Aldrich), amiloride (10 –3 M, 300 μl intratracheally; Sigma-Aldrich), or zinterol (10 –7 M, 300 μl intratracheally; Tocris); or vehicle ( 13 ).
Article Title: A2B adenosine receptor signaling attenuates acute lung injury by enhancing alveolar fluid clearance in mice
Article Snippet: In subsets of experiments, mice were treated with the A2BAR antagonist PSB1115 (10 mg/kg i.p.; Tocris) or the A2BAR agonist BAY 60-6583 (2 mg/kg i.p.; Bayer Healthcare), the A1AR antagonist DPCPX (1 mg/kg i.p.; Tocris), the A3AR antagonist MRS1191 (1 mg/kg i.p.; Sigma-Aldrich), amiloride (10–3 M, 300 μl intratracheally; Sigma-Aldrich), or zinterol (10–7 M, 300 μl intratracheally; Tocris); or vehicle (13).
Quantitative RT-PCR:Article Title: A2B adenosine receptor signaling attenuates acute lung injury by enhancing alveolar fluid clearance in mice
Article Snippet: In subsets of experiments, mice were treated with the A2BAR antagonist PSB1115 (10 mg/kg i.p.; Tocris) or the A2BAR agonist BAY 60-6583 (2 mg/kg i.p.; Bayer Healthcare), the A1AR antagonist DPCPX (1 mg/kg i.p.; Tocris), the A3AR antagonist MRS1191 (1 mg/kg i.p.; Sigma-Aldrich), amiloride (10 –3 M, 300 μl intratracheally; Sigma-Aldrich), or zinterol (10 –7 M, 300 μl intratracheally; Tocris); or vehicle ( 13 ).
Article Title: A2B adenosine receptor signaling attenuates acute lung injury by enhancing alveolar fluid clearance in mice
Article Snippet: In subsets of experiments, mice were treated with the A2BAR antagonist PSB1115 (10 mg/kg i.p.; Tocris) or the A2BAR agonist BAY 60-6583 (2 mg/kg i.p.; Bayer Healthcare), the A1AR antagonist DPCPX (1 mg/kg i.p.; Tocris), the A3AR antagonist MRS1191 (1 mg/kg i.p.; Sigma-Aldrich), amiloride (10–3 M, 300 μl intratracheally; Sigma-Aldrich), or zinterol (10–7 M, 300 μl intratracheally; Tocris); or vehicle (13).
Gene Expression:Article Title: A2B adenosine receptor signaling attenuates acute lung injury by enhancing alveolar fluid clearance in mice
Article Snippet: In subsets of experiments, mice were treated with the A2BAR antagonist PSB1115 (10 mg/kg i.p.; Tocris) or the A2BAR agonist BAY 60-6583 (2 mg/kg i.p.; Bayer Healthcare), the A1AR antagonist DPCPX (1 mg/kg i.p.; Tocris), the A3AR antagonist MRS1191 (1 mg/kg i.p.; Sigma-Aldrich), amiloride (10 –3 M, 300 μl intratracheally; Sigma-Aldrich), or zinterol (10 –7 M, 300 μl intratracheally; Tocris); or vehicle ( 13 ).
Article Title: A2B adenosine receptor signaling attenuates acute lung injury by enhancing alveolar fluid clearance in mice
Article Snippet: In subsets of experiments, mice were treated with the A2BAR antagonist PSB1115 (10 mg/kg i.p.; Tocris) or the A2BAR agonist BAY 60-6583 (2 mg/kg i.p.; Bayer Healthcare), the A1AR antagonist DPCPX (1 mg/kg i.p.; Tocris), the A3AR antagonist MRS1191 (1 mg/kg i.p.; Sigma-Aldrich), amiloride (10–3 M, 300 μl intratracheally; Sigma-Aldrich), or zinterol (10–7 M, 300 μl intratracheally; Tocris); or vehicle (13).
Real-time Polymerase Chain Reaction:Article Title: A2B adenosine receptor signaling attenuates acute lung injury by enhancing alveolar fluid clearance in mice
Article Snippet: In subsets of experiments, mice were treated with the A2BAR antagonist PSB1115 (10 mg/kg i.p.; Tocris) or the A2BAR agonist BAY 60-6583 (2 mg/kg i.p.; Bayer Healthcare), the A1AR antagonist DPCPX (1 mg/kg i.p.; Tocris), the A3AR antagonist MRS1191 (1 mg/kg i.p.; Sigma-Aldrich), amiloride (10 –3 M, 300 μl intratracheally; Sigma-Aldrich), or zinterol (10 –7 M, 300 μl intratracheally; Tocris); or vehicle ( 13 ).
Article Title: A2B adenosine receptor signaling attenuates acute lung injury by enhancing alveolar fluid clearance in mice
Article Snippet: In subsets of experiments, mice were treated with the A2BAR antagonist PSB1115 (10 mg/kg i.p.; Tocris) or the A2BAR agonist BAY 60-6583 (2 mg/kg i.p.; Bayer Healthcare), the A1AR antagonist DPCPX (1 mg/kg i.p.; Tocris), the A3AR antagonist MRS1191 (1 mg/kg i.p.; Sigma-Aldrich), amiloride (10–3 M, 300 μl intratracheally; Sigma-Aldrich), or zinterol (10–7 M, 300 μl intratracheally; Tocris); or vehicle (13).
Expressing:Article Title: A2B adenosine receptor signaling attenuates acute lung injury by enhancing alveolar fluid clearance in mice
Article Snippet: In subsets of experiments, mice were treated with the A2BAR antagonist PSB1115 (10 mg/kg i.p.; Tocris) or the A2BAR agonist BAY 60-6583 (2 mg/kg i.p.; Bayer Healthcare), the A1AR antagonist DPCPX (1 mg/kg i.p.; Tocris), the A3AR antagonist MRS1191 (1 mg/kg i.p.; Sigma-Aldrich), amiloride (10 –3 M, 300 μl intratracheally; Sigma-Aldrich), or zinterol (10 –7 M, 300 μl intratracheally; Tocris); or vehicle ( 13 ).
Article Title: A2B adenosine receptor signaling attenuates acute lung injury by enhancing alveolar fluid clearance in mice
Article Snippet: In subsets of experiments, mice were treated with the A2BAR antagonist PSB1115 (10 mg/kg i.p.; Tocris) or the A2BAR agonist BAY 60-6583 (2 mg/kg i.p.; Bayer Healthcare), the A1AR antagonist DPCPX (1 mg/kg i.p.; Tocris), the A3AR antagonist MRS1191 (1 mg/kg i.p.; Sigma-Aldrich), amiloride (10–3 M, 300 μl intratracheally; Sigma-Aldrich), or zinterol (10–7 M, 300 μl intratracheally; Tocris); or vehicle (13).
SDS Page:Article Title: A2B adenosine receptor signaling attenuates acute lung injury by enhancing alveolar fluid clearance in mice
Article Snippet: In subsets of experiments, mice were treated with the A2BAR antagonist PSB1115 (10 mg/kg i.p.; Tocris) or the A2BAR agonist BAY 60-6583 (2 mg/kg i.p.; Bayer Healthcare), the A1AR antagonist DPCPX (1 mg/kg i.p.; Tocris), the A3AR antagonist MRS1191 (1 mg/kg i.p.; Sigma-Aldrich), amiloride (10 –3 M, 300 μl intratracheally; Sigma-Aldrich), or zinterol (10 –7 M, 300 μl intratracheally; Tocris); or vehicle ( 13 ).
Article Title: A2B adenosine receptor signaling attenuates acute lung injury by enhancing alveolar fluid clearance in mice
Article Snippet: In subsets of experiments, mice were treated with the A2BAR antagonist PSB1115 (10 mg/kg i.p.; Tocris) or the A2BAR agonist BAY 60-6583 (2 mg/kg i.p.; Bayer Healthcare), the A1AR antagonist DPCPX (1 mg/kg i.p.; Tocris), the A3AR antagonist MRS1191 (1 mg/kg i.p.; Sigma-Aldrich), amiloride (10–3 M, 300 μl intratracheally; Sigma-Aldrich), or zinterol (10–7 M, 300 μl intratracheally; Tocris); or vehicle (13).
Western Blot:Article Title: A2B adenosine receptor signaling attenuates acute lung injury by enhancing alveolar fluid clearance in mice
Article Snippet: In subsets of experiments, mice were treated with the A2BAR antagonist PSB1115 (10 mg/kg i.p.; Tocris) or the A2BAR agonist BAY 60-6583 (2 mg/kg i.p.; Bayer Healthcare), the A1AR antagonist DPCPX (1 mg/kg i.p.; Tocris), the A3AR antagonist MRS1191 (1 mg/kg i.p.; Sigma-Aldrich), amiloride (10 –3 M, 300 μl intratracheally; Sigma-Aldrich), or zinterol (10 –7 M, 300 μl intratracheally; Tocris); or vehicle ( 13 ).
Article Title: A2B adenosine receptor signaling attenuates acute lung injury by enhancing alveolar fluid clearance in mice
Article Snippet: In subsets of experiments, mice were treated with the A2BAR antagonist PSB1115 (10 mg/kg i.p.; Tocris) or the A2BAR agonist BAY 60-6583 (2 mg/kg i.p.; Bayer Healthcare), the A1AR antagonist DPCPX (1 mg/kg i.p.; Tocris), the A3AR antagonist MRS1191 (1 mg/kg i.p.; Sigma-Aldrich), amiloride (10–3 M, 300 μl intratracheally; Sigma-Aldrich), or zinterol (10–7 M, 300 μl intratracheally; Tocris); or vehicle (13).
Clinical Proteomics:Article Title: A2B adenosine receptor signaling attenuates acute lung injury by enhancing alveolar fluid clearance in mice
Article Snippet: In subsets of experiments, mice were treated with the A2BAR antagonist PSB1115 (10 mg/kg i.p.; Tocris) or the A2BAR agonist BAY 60-6583 (2 mg/kg i.p.; Bayer Healthcare), the A1AR antagonist DPCPX (1 mg/kg i.p.; Tocris), the A3AR antagonist MRS1191 (1 mg/kg i.p.; Sigma-Aldrich), amiloride (10 –3 M, 300 μl intratracheally; Sigma-Aldrich), or zinterol (10 –7 M, 300 μl intratracheally; Tocris); or vehicle ( 13 ).
Article Title: A2B adenosine receptor signaling attenuates acute lung injury by enhancing alveolar fluid clearance in mice
Article Snippet: In subsets of experiments, mice were treated with the A2BAR antagonist PSB1115 (10 mg/kg i.p.; Tocris) or the A2BAR agonist BAY 60-6583 (2 mg/kg i.p.; Bayer Healthcare), the A1AR antagonist DPCPX (1 mg/kg i.p.; Tocris), the A3AR antagonist MRS1191 (1 mg/kg i.p.; Sigma-Aldrich), amiloride (10–3 M, 300 μl intratracheally; Sigma-Aldrich), or zinterol (10–7 M, 300 μl intratracheally; Tocris); or vehicle (13).
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